【#第一文档网# 导语】以下是®第一文档网的小编为您整理的《实验报告(1)(1)》，欢迎阅读！
Pathogen isolation and identification of clinical specimens Ⅰ Shape, chromaticity ,growth of cocci in blood agar and enterobacteriaceae in EMB Material: (1)Specimens: pus swab, throat swab,stood-1,stood-2,urine (2)Culture mediums：Blood agar, EMB, (3)Other:Gram stain regents Method: colony observation: Take pus swab and throat swab ,using four-area streak to inoculate in the blood agar.37° cultivation morphology observation: Take single colony smearing, Gram stain, microscopic examination. Result record-1 Gram stain microscopic examination Blood agar result observation Shape arrangement chromaticity colony feature hemolysis colour pus swab (Bacteria-1) throat swab (Bacteria-2&3) Sphere Staphylo (+) small, circle β-hemolysis aureus Sphere Chains (+) small, circle α- hemolysis white Sphere Chains (+) big, circle α- hemolysis white Result record-2 Colony shape on the EMB Urine(Bacteria-4) small, Purple or dark purple and metal luster Stood-1 small, Purple or dark purple and metal luster& smaller, (Bacteria-4&5) colourless and subtransparent Stood-2 small, Purple or dark purple and metal luster& smaller, (Bacteria-4&6) colourless and subtransparent Discussion (1)pus swab may have S.aureus (2)throat swab may have S.pneumoniae and α-streptococcus (3)urine may have E.coli (4)stood-1 may have E.coli and other enterobacteriaceae (5)stood-2 may have E.coli and other enterobacteriaceae Ⅱ Plasma coagulase test & Catelase test Material: (1)specimens: Bacteria-1 (2) reagent: NS, rabbit plasma, 3% H2O2 Method: Plasma coagulase test:(1)Add one drop of NS for each side (2)Add a single colony of Bacteria-1 for each side with the loop (3)Add one drop of rabbit plasma for the left side (4)Shaking gently and observe the appearance at the same time Catalase test: (1)Add one drop of NS for each side (2)Add a single colony of Bacteria-1 for right side with the loop (3) Add 3% H2O2 for each side and observe the appearance Result record: Bacteria-1 Plasma coagulase test left(+) right(-) Catelase test left(-) right(+) Discussion (1) Bacteria-1 is S.aureus Ⅲ Capsule stain, Bile solubility test, Optochin test of Streptococcus Material: (1)specimens: Bacteria-2&3 (2)reagent: Gram stain regents, 10% bile salt, Optochin Method: Capsule staining:(1)Make smear, air dry, heat fix (2)Add 1 drop of carbol fuchsin, immediately flush away (3)Observe under microscope Bile solubility test:(1)Labeled single colonies on blood agar (2)Directly add a drop of deoxycholic acid sodium solution on the colony (3)The plate is incubated at 37℃ for 20-30 minutes (4)Observation Optochin test:(1)Pick single colony smearing on blood agar, adding optochin paper on the inoculation (2)The plate is incubated at 37℃ for 18-24 hours (3)Observation Result record: Capsule stain: Bacteria-3 surrounded by pink capsule Bile solubility test: Bacteria-3 dissolved by bile salt Optochin test: the diameter of bacteriostasis circle greater than 20mm is Bacteria-3 the diameter of bacteriostasis circle greater than 20mm is Bacteria-2 Discussion Bacteria-2 maybe α-streptococcus Bacteria-3 is S. pneumoniae IV Biochemical reaction of enterobacteriaceae Material: (1)specimens:Bacteria-4&5&6 (2)culture medium: Double sugar iron slant, Indole urea semisolid medium (3)reagent: Kovac’s reagent Method: DSI reaction test:(1)Take three specimens streak slant surface and stab into DSI (2)37℃ cultivation 24 hours MIU test:(1) Take three specimens stab into MIU (2) 37℃ cultivation 24 hours (3)Add 3~4 drops Kovac’s reagent in each tube, Result record-1(DSI) slant butt gas H2S Bacteria-4 yellow yellow (+) (-) motivity (+) (+) (-) Bacteria-5 red yellow (-) (+) indole (+) (-) (-) Bacteria-6 red yellow (-) (-) urease (-) (-) (-) Result record-2(MIU) Bacteria-4 Bacteria-5 Bacteria-6 Discussion (1)Bacteria-4 is E.coli (2)Bacteria-5 is S.typhi (3)Bacteria-6 is S.dysenteriae V Slide agglutination test Material: (1)specimens: S.typhi& S.dysenteriae (2)diagnostic sera: S.typhi diagnostic sera Method: (1)Divide the slide into two equal parts (2)Add 1 drop of NS on each side (3)Emulsify S.typhi in the left side and S.dysenteriae in the right side (4)Add one drop of diagnostic sera for S.typhi on the left side, and then on the right side (5)Gently shake the slide and observe the result Result record phenomenon left (+) right (-) The left side agglutination while the right side no phenomenon 本文来源：https://www.dywdw.cn/3cbc930111661ed9ad51f01dc281e53a58025198.html
下载此文档
搜索文档
阅读：
文档下载
2022-04-01
